Results obtained in this study will be helpful for cloning and analysis of drought-resistant genes of Jerusalem artichoke in the future, and also lay the foundation for the explanation of the drought resistance mechanism of Jerusalem artichoke and are helpful for the identification of drought tolerant Jerusalem artichoke varieties. These findings suggest that transcription factors may respond to drought stress by regulating structural genes expression level in metabolic pathways and then regulating the accumulation of related metabolites. A transcription factor analysis showed that NAC, MYB, WRKY, homeobox-leucine zipper ( HD-ZIP) and basic leucine-zipper ( bZIP) were considered to be closely related to the response to PEG-simulated drought stress. Kyoto Encyclopedia of Genes and Genomes enrichment showed that differential expression genes were mainly classified in metabolic pathways biosynthesis of secondary metabolites photosynthesis - antenna proteins circadian rhythm-plant porphyrin and chlorophyll metabolism phenylpropanoid biosynthesis ABC transporters flavonoid biosynthesis carotenoid biosynthesis stilbenoid, diarylheptanoid, and gingerol biosynthesis cutin, suberin, and wax biosynthesis and isoquinoline alkaloid biosynthesis. Gene Ontology enrichment showed that differential expression genes were classified in plastoglobules, photosystem II, and photosystem. Artichokescan be tricky, but with the help of this growing guide you can havesuccess even a. A total number of 172,291 unigenes were generated from the leaves of Jerusalem artichoke and 17,612 unigenes were identified as differentially expressed unigenes. In this episode, we will be talking about how to grow artichokes. In this study, Jerusalem artichoke seedlings under polyethylene glycol (PEG) 6000-simulated drought stress were used for analysis of malonaldehyde, proline, and soluble sugar content, then the differentially expressed unigenes were identified by RNA-sequencing. Step 3: Know when to vernalize (that ‘fake winter’ thing) Your artichoke seedlings will need to grow under lights (or in a warm greenhouse) for at least 8 to 10 weeks, as you’ll want to give them enough time to form a few pairs of full-size leaves. Jerusalem artichoke ( Helianthus tuberosus L.) is a perennial crop with high drought tolerance that can be used in ecological management. Keep seed trays warm (65F) under grow lights or in a greenhouse. Drought stress is one of the most important abiotic stresses affecting crop growth and development, which leads to huge production and economics losses.
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